Established 2026 · Free Educational Resources for All

Biology · 100-Mark Comprehensive Assessment

Biology Comprehensive Practice Test 3

A 100-mark Biology assessment covering 0-0-5 — What Is the Human Cell? A Simple Guide, 0-0-6 — Biological Molecules, Enzymes & Food Tests — progressing from foundational knowledge to application, analysis and higher-order reasoning.

EDUSAMBAM Editorial Team·45 questions · 100 marks·Basic → Advanced

Questions

100

Marks

Sections

100%

Auto-Graded

A — Multiple Choice	15	2	30
B — True / False	10	2	20
C — Fill in the Blank	10	2	20
D — Column Matching	5 pairs	2	10
E — Short Answer	5	4	20
Total	45	—	100
Total	45	—	100

Fill-in-the-blank and short-answer questions are checked automatically against accepted answers — exact wording does not need to match, but the key term should be present.

0 of 45 answered

0/100

You scored 0%

Keep practicing

Section A — Multiple Choice

30 marks

15 questions · 2 marks each.

1.Which organelle contains most of a eukaryotic cell's DNA?Basic2 pts

A. Nucleus

B. Ribosome

C. Lysosome

D. Golgi apparatus

2.Ribosomes are directly involved in:Basic2 pts

A. Protein synthesis

B. DNA storage

C. Lipid storage only

D. Cell wall digestion

3.The plasma membrane is mainly a:Basic2 pts

A. Selective boundary containing a lipid bilayer and proteins

B. Rigid cellulose wall in all cells

C. DNA layer

D. Protein-free barrier

4.Which molecule is a polymer made from amino acids?Basic2 pts

A. Protein

B. Starch

C. DNA

D. Triglyceride

5.Enzymes accelerate reactions primarily by:Basic2 pts

A. Lowering activation energy

B. Increasing the reaction's overall energy change

C. Being consumed as reactants

D. Raising every reaction temperature

6.A very high temperature can reduce enzyme activity because:Intermediate2 pts

A. Protein structure can be disrupted, changing the active site

B. All substrates disappear

C. ATP becomes DNA

D. Water becomes a carbohydrate

7.The iodine test is commonly used to detect:Intermediate2 pts

A. Starch

B. Protein

C. Lipids only

D. Vitamin C

8.A positive Benedict's test after heating can indicate:Intermediate2 pts

A. Reducing sugars

B. Starch only

C. Protein only

D. DNA only

9.The Biuret test is associated with:Intermediate2 pts

A. Peptide bonds in proteins

B. Starch granules

C. Lipids only

D. Mineral ions

10.The emulsion test is commonly used for:Intermediate2 pts

A. Lipids

B. Starch

C. Protein

D. Reducing sugars

11.If an enzyme's active site changes shape, the most direct consequence is likely:Advanced2 pts

A. Reduced ability to bind or convert its substrate

B. Immediate increase in all reactions

C. Creation of a new organelle

D. Loss of all DNA

12.Which statement best describes hydrolysis?Advanced2 pts

A. A bond is broken using water, often splitting a larger molecule

B. Two monomers always join without water

C. A protein becomes DNA

D. Energy is never involved

13.Why are controls important in food tests?Advanced2 pts

A. They help show that observed colour changes are associated with the test procedure and sample

B. They make every sample positive

C. They eliminate the need for reagents

D. They prove exact concentration

14.A positive qualitative food test generally tells you that:Advanced2 pts

A. The target substance or reactive group is detected under the test conditions

B. The exact concentration is known

C. The sample is healthy

D. Every related molecule is present

15.Which statement best connects structure and function?Advanced2 pts

A. Molecular structure influences interactions, so changing structure can alter biological function

B. Structure never affects function

C. Only size determines function

D. Function is independent of molecular shape

Section B — True / False

20 marks

10 questions · 2 marks each.

16.All proteins are enzymes.Basic2 pts

True

False

17.Enzymes are generally unchanged at the end of the reactions they catalyse.Basic2 pts

True

False

18.A negative iodine test proves that a food contains no carbohydrates of any type.Basic2 pts

True

False

19.The cell membrane helps maintain different conditions inside and outside the cell.Basic2 pts

True

False

20.DNA and RNA are nucleic acids.Basic2 pts

True

False

21.Lipids can contribute to membranes and energy storage.Intermediate2 pts

True

False

22.A qualitative test always gives an exact concentration.Intermediate2 pts

True

False

23.Substrate concentration can affect enzyme reaction rate under suitable conditions.Advanced2 pts

True

False

24.Extreme pH can alter the structure and activity of many enzymes.Advanced2 pts

True

False

25.Controls are unnecessary when comparing two food samples.Advanced2 pts

True

False

Section C — Fill in the Blank

20 marks

10 questions · 2 marks each. Type the missing word or term.

26.The monomers that form proteins are ______ acids.Intermediate2 pts

27.The enzyme region where a substrate binds is the ______ site.Intermediate2 pts

28.The main carbohydrate storage polymer in plants is ______.Intermediate2 pts

29.The iodine solution test is commonly used to detect ______.Intermediate2 pts

30.Benedict's reagent can indicate the presence of ______ sugars.Intermediate2 pts

31.The Biuret test is used to detect ______.Advanced2 pts

32.Lipids can be detected using the ethanol ______ test.Advanced2 pts

33.A reaction that breaks a molecule using water is called ______.Advanced2 pts

34.The selectively permeable boundary around a cell is the plasma ______.Advanced2 pts

35.The molecule that stores most hereditary information in cells is ______.Advanced2 pts

Section D — Column Matching

10 marks

Use the ▲ ▼ buttons to reorder Column B until each row lines up with the correct term in Column A. 2 marks per correct pair.

36–40.Match each term in Column A with the correct description in Column B.Advanced10 pts

Column A

Carbohydrate

Lipid

Protein

Iodine test

Biuret test

Column B

B. Membranes and energy storage

C. Structure, transport, catalysis and signalling

D. Starch detection

E. Protein detection

A. Energy supply or structural roles

Section E — Short Answer

20 marks

5 questions · 4 marks each. Answer in one or two sentences.

41.Explain why enzyme activity depends on three-dimensional protein structure.Advanced4 pts

42.A food gives a positive Benedict's test but a negative iodine test. What can reasonably be concluded?Advanced4 pts

43.Why is a control important when carrying out a biochemical food test?Advanced4 pts

44.Distinguish hydrolysis from condensation using biological molecules as examples.Advanced4 pts

45.Explain how membrane structure supports selective transport.Advanced4 pts

← Gateway